Myosin cooperates with microtubule motors during organelle transport in melanophores

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Myosin cooperates with microtubule motors during organelle transport in melanophores

Melanophores offer an outstanding system for the study of intracellular motility. These cells aggregate their pigment-filled melanosomes to the cell center or disperse them throughout the cytoplasm in response to hormonal modulation of intracellular cyclic AMP levels in order to effect color changes in lower vertebrates [1]. Previous work from our laboratory demonstrated a role for microtubule-...

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Organelle Transport: Dynamic Actin Tracks for Myosin Motors

Transport of cargo by molecular motors on microtubule and actin filament tracks is a fundamental property of eukaryotic cells. A new study reports that actin dynamics are required in cells for myosin I and V motor proteins to transport their organelle cargos on actin tracks.

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Regulation of organelle transport in melanophores by calcineurin

Previous studies have shown that pigment granule dispersion and aggregation in melanophores of the African cichlid, Tilapia mossambica, are regulated by protein phosphorylation and dephosphorylation, respectively (Rozdzial, M. M., and L. T. Haimo. 1986. Cell. 47:1061-1070). The present studies suggest that calcineurin, a Ca2+/calmodulin-stimulated phosphatase, is the endogenous phosphatase that...

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Multiscale trend analysis of microtubule transport in melanophores.

Microtubule-based transport is critical for trafficking of organelles, organization of endomembranes, and mitosis. The driving force for microtubule-based transport is provided by microtubule motors, which move organelles specifically to the plus or minus ends of the microtubules. Motor proteins of opposite polarities are bound to the surface of the same cargo organelle. Transport of organelles...

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We have studied the dynamics of microtubules in black tetra (Gymnocorymbus ternetzi) melanophores to test the possible correlation of microtubule stability and intracellular particle transport. X-rhodamine-or caged fluorescein-conjugated tubulin were microinjected and visualized by fluorescence digital imaging using a cooled charge coupled device and videomicroscopy. Microtubule dynamics were e...

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ژورنال

عنوان ژورنال: Current Biology

سال: 1998

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(98)70063-6